This kit has been developed as a reagent for research purposes to detect respective subtypic determinants, d, y, r, and w, in hepatitis B surface antigen (HBsAg) positive samples for identification of HBsAg subtypes such as adr, adw, ayr and ayw.

Detection of subtypic determinants is based on the solid-phase sandwich EIA (Enzyme Immuno Assay). 96 wells of the microplate are coated with monoclonal antibody against the common determinant a of HBsAg. HBsAg in samples dispensed to wells is caught on the solid phase and their subtypic determinant, d, y, r, or w, is detected by peroxidase-labeled monoclonal antibody against corresponding determinant.