{"id":1007,"date":"2017-08-10T10:00:00","date_gmt":"2017-08-10T01:00:00","guid":{"rendered":"http:\/\/iij.webpacklab.com\/wp\/?page_id=929"},"modified":"2024-11-27T15:24:31","modified_gmt":"2024-11-27T06:24:31","slug":"ko","status":"publish","type":"page","link":"https:\/\/www.tokumen.co.jp\/en\/cell\/ko\/","title":{"rendered":"Gene Targeting"},"content":{"rendered":"<div id=\"keybox\">\n<div class=\"title title_cat2\">\n<h2>Authentic Gene Targeting<\/h2>\n<\/p><\/div>\n<div id=\"breadcrumb\">\n<nav class=\"single\">\n<ol>\n<li><a href=\"\/en\/\">HOME<\/a><\/li>\n<li><a href=\"\/en\/cell\/\">GE Services<\/a><\/li>\n<li>Authentic Gene Targeting<\/li>\n<\/ol>\n<\/nav><\/div>\n<\/div>\n<p><main role=\"main\"><\/p>\n<article id=\"pages_area\">\n<section class=\"pages inviewUp\">\n<div class=\"cntbox\">\n<h2>Authentic Gene Targeting<\/h2>\n<h3>Contract generation of knockin \/ knockout mice<\/h3>\n<div class=\"textonly\">\n<p>\n                    We will provide you a whole range of services for knockin mice generation, including targeting vector construction, mouse ES cell transfection, homologous recombinant screening and confirmation, chimeric mice production, F1 mice production, selection marker deletion from genome by Cre or Flp, and heterozygous mutant selection.<br \/>\n                    You can take an advantage of our outstanding gene construction by BAC recombineering.<br \/>\n                    We don\u2019t amplify any genomic sequences by PCR, but clone every homology arm and inserted gene elements by Red\/ET recombination. This enables high efficient and dramatically precise gene targeting, which results in functional gene insertion or replacement such as a mutant sequence, a reporter gene, loxP sequences or human genes. Because we carefully identify the targeted gene locus by Southern blotting, you can avoid frequently occurring non-homologous mutant mice.\n                <\/p>\n<\/p><\/div>\n<div class=\"imgonly\">\n<div class=\"imgbox\">\n                        <!-- <span>K1_\u56f32<\/span> --><br \/>\n                        <img decoding=\"async\" src=\"https:\/\/www.tokumen.co.jp\/wp\/wp-content\/themes\/iij_custom\/images\/2023_re\/page\/img_d3_01_03_en.svg\" alt=\"K1_2\">\n                    <\/div>\n<\/p><\/div>\n<p>                <!-- \n\n<h2>\u30ce\u30c3\u30af\u30a2\u30a6\u30c8\u30de\u30a6\u30b9<\/h2>\n\n\n                \n\n<h3>\u30ce\u30c3\u30af\u30a2\u30a6\u30c8\u30de\u30a6\u30b9\u4f5c\u88fd\u53d7\u8a17\u30b5\u30fc\u30d3\u30b9\u5185\u5bb9<\/h3>\n\n\n                \n\n<div class=\"textonly\">\n                    \n\n<p>\n                        Red\/ET \u30c6\u30af\u30ce\u30ed\u30b8\u30fc\u3092\u5229\u7528\u3057\u3066\u3001\u6a19\u7684\u907a\u4f1d\u5b50\u306e\u30ce\u30c3\u30af\u30a2\u30a6\u30c8\u30bf\u30fc\u30b2\u30c6\u30a3\u30f3\u30b0\u30d9\u30af\u30bf\u30fc\u3092\u69cb\u7bc9\u3057\u3001\u907a\u4f1d\u5b50\u30bf\u30fc\u30b2\u30c6\u30a3\u30f3\u30b0\u306b\u3088\u308a\u6a19\u7684\u907a\u4f1d\u5b50\u306e\u69cb\u6210\u907a\u4f1d\u5b50\u304c\u6b20\u640d\u3057\u305f ES \u7d30\u80de\u682a\u3092\u6a39\u7acb\u81f4\u3057\u307e\u3059\u3002\u305d\u306e ES \u7d30\u80de\u682a\u3092\u30de\u30a6\u30b9\u521d\u671f\u80da\u306b\u6ce8\u5165\u3057\u3066\u30ad\u30e1\u30e9 \u30de\u30a6\u30b9\u3092\u4f5c\u51fa\u3057\u3001\u3055\u3089\u306b\u30ad\u30e1\u30e9\u30de\u30a6\u30b9\u3068\u91ce\u751f\u578b\u30de\u30a6\u30b9\u3092\u4ea4\u914d\u3055\u305b\u3066\u7523\u5b50\u3092\u5f97\u3066\u3001\u6ce8\u5165\u3057\u305f ES \u7d30\u80de\u304b\u3089\u5206\u5316\u3057\u305f\u7cbe\u5b50\u306b\u7531\u6765\u3059\u308b F1\u30de\u30a6\u30b9\u3092\u9078\u629e\u81f4\u3057\u307e\u3059\u3002\u3053\u308c\u3089\u306e F1 \u30de\u30a6\u30b9\u306e\u3046\u3061\u3001\u69cb\u6210\u907a\u4f1d\u5b50\u304c\u6b20\u640d\u3057\u305f\u6a19\u7684\u907a\u4f1d\u5b50\u5ea7\u3092\u6301\u3064\u30d8\u30c6\u30ed\u30df\u30e5\u30fc\u30bf\u30f3\u30c8\u30de\u30a6\u30b9\u3092\u9078\u629e\u3057\u307e\u3059\u3002\n                    <\/p>\n\n\n                <\/div>\n\n --><\/p>\n<div class=\"imgimg flex-img\">\n<div class=\"imgbox\"><!-- <span>KO_1<\/span> --><img decoding=\"async\" src=\"https:\/\/www.tokumen.co.jp\/wp\/wp-content\/themes\/iij_custom\/images\/2023_re\/page\/img_d3_01_04_en.svg\" alt=\"KO_1\"><\/div>\n<div class=\"imgbox\"><!-- <span>KO_2<\/span> --><img decoding=\"async\" src=\"https:\/\/www.tokumen.co.jp\/wp\/wp-content\/themes\/iij_custom\/images\/pages\/img_d3_01_05_en.png\" alt=\"KO_2\"><\/div>\n<\/p><\/div>\n<\/p><\/div>\n<\/section>\n<\/article>\n<p><\/main><\/p>\n","protected":false},"excerpt":{"rendered":"<p>Authentic Gene Targeting HOME GE Services Authentic Gene Targeting Authentic Gene Targeting Contract generation of knockin \/ knockout mice We will provide you a whole range of services for knockin mice generation, including targeting vector construction, mouse ES cell transfection, homologous recombinant screening and confirmation, chimeric mice production, F1 mice production, selection marker deletion from [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"parent":1004,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_acf_changed":false,"_locale":"en_US","_original_post":"34","footnotes":""},"class_list":["post-1007","page","type-page","status-publish","hentry","en-US"],"acf":[],"aioseo_notices":[],"_links":{"self":[{"href":"https:\/\/www.tokumen.co.jp\/wp-json\/wp\/v2\/pages\/1007"}],"collection":[{"href":"https:\/\/www.tokumen.co.jp\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/www.tokumen.co.jp\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/www.tokumen.co.jp\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.tokumen.co.jp\/wp-json\/wp\/v2\/comments?post=1007"}],"version-history":[{"count":9,"href":"https:\/\/www.tokumen.co.jp\/wp-json\/wp\/v2\/pages\/1007\/revisions"}],"predecessor-version":[{"id":3810,"href":"https:\/\/www.tokumen.co.jp\/wp-json\/wp\/v2\/pages\/1007\/revisions\/3810"}],"up":[{"embeddable":true,"href":"https:\/\/www.tokumen.co.jp\/wp-json\/wp\/v2\/pages\/1004"}],"wp:attachment":[{"href":"https:\/\/www.tokumen.co.jp\/wp-json\/wp\/v2\/media?parent=1007"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}